作业帮跪求高手翻译呀,别用翻译软件呀,分较少呀,先谢谢了
来源：学生作业帮 编辑：作业帮 分类：英语作业 时间：2024/07/14 15:20:33
跪求高手翻译呀,别用翻译软件呀,分较少呀,先谢谢了
The breakdown of cellular comets for our proposed method of image analysis is shown schematically in Figure 2. All methods in current use employ some technique to separate the comet into its component head and tail. For example, Tice et al. (1990) measured the cell diameter perpendicular to the direction of electrophoresis to define the comet head. In the present method the optical density of the whole comet is integrated perpendicular to the direction of electrophoresis and the 'intact' cell is estimated by reflection of the leading edge of the cell into the tail. The tail DNA is then derived by subtraction of the 'intact' cell from the whole comet (Figure2). This one-dimensional analysis of fluorescence improves the signal-to-noise ratio over the original method of Tice et al.(1990), and is currently used by those investigators. Although the fraction of DNA in the comet tail is itself a valid assay parameter, most investigations also take account of the length of the comet tail and derive the composite parameter 'tail moment'. The tail moment has come into use with the comet assay by analogy with the mechanical engineering term distance from an axis (Random House, 1987). Thus, given the possibility both of a short tail containing a high fraction of DNA, or of a long tail containing a low fraction of highly fragmented DNA, a function of the product of these two variables became an attractive assay parameter. Hitherto, the tail moment has been calculated by multiplying the fraction of DNA in the tail by half the length of the tail (e.g. Olive et al. 1990a,b; Tice et al., 1990). Several methods of determining the tail length have been used. Singh et al. (1988) measured the length of the tail from the leading edge of the cell (l" in Figure 2). Olive et al. (1990a,b) and others ,commence tail measurements from the estimated centre of the cell (/' in Figure 2). Both of these measurements mean that a cell without a DNA tail is recorded as having a tail of length equal to that of the cellular diameter, or radius, respectively. This, in turn, leads to intact cells having a positive tail moment. We considered that this might complicate the discernment and statistical analysis of weak induced effects, so the image analysis program was modified to commence measurement of the tail at the estimated trailing edge of the cell (l in Figure 2). We also decided to omit the denominator of 2 from the calculation of comet tail moment because the tail is not of uniform shape. It is therefore proposed (and done herein) that comet tail moment should be the product of the length of the comet tail (measured from the trailing edge of the cell) and the fraction of DNA in the tail. The 2 h control data are shown in Figure 3 using the centre and the trailing edge tail length measures.
The breakdown of cellular comets for our proposed method of image analysis is shown schematically in Figure 2. All methods in current use employ some technique to separate the comet into its component head and tail. For example, Tice et al. (1990) measured the cell diameter perpendicular to the direction of electrophoresis to define the comet head. In the present method the optical density of the whole comet is integrated perpendicular to the direction of electrophoresis and the 'intact' cell is estimated by reflection of the leading edge of the cell into the tail. The tail DNA is then derived by subtraction of the 'intact' cell from the whole comet (Figure2). This one-dimensional analysis of fluorescence improves the signal-to-noise ratio over the original method of Tice et al.(1990), and is currently used by those investigators. Although the fraction of DNA in the comet tail is itself a valid assay parameter, most investigations also take account of the length of the comet tail and derive the composite parameter 'tail moment'. The tail moment has come into use with the comet assay by analogy with the mechanical engineering term distance from an axis (Random House, 1987). Thus, given the possibility both of a short tail containing a high fraction of DNA, or of a long tail containing a low fraction of highly fragmented DNA, a function of the product of these two variables became an attractive assay parameter. Hitherto, the tail moment has been calculated by multiplying the fraction of DNA in the tail by half the length of the tail (e.g. Olive et al. 1990a,b; Tice et al., 1990). Several methods of determining the tail length have been used. Singh et al. (1988) measured the length of the tail from the leading edge of the cell (l" in Figure 2). Olive et al. (1990a,b) and others ,commence tail measurements from the estimated centre of the cell (/' in Figure 2). Both of these measurements mean that a cell without a DNA tail is recorded as having a tail of length equal to that of the cellular diameter, or radius, respectively. This, in turn, leads to intact cells having a positive tail moment. We considered that this might complicate the discernment and statistical analysis of weak induced effects, so the image analysis program was modified to commence measurement of the tail at the estimated trailing edge of the cell (l in Figure 2). We also decided to omit the denominator of 2 from the calculation of comet tail moment because the tail is not of uniform shape. It is therefore proposed (and done herein) that comet tail moment should be the product of the length of the comet tail (measured from the trailing edge of the cell) and the fraction of DNA in the tail. The 2 h control data are shown in Figure 3 using the centre and the trailing edge tail length measures.
.忙着写论文把翻译的事儿都忘了.
再问： 啥也不说了，内牛满面呀！！
再答： 我们对细胞彗星的分解是依据所提出的图像分析法进行的，详见表2。现今用到的所有分析方法均需将彗星分解为慧头与慧尾。 1990年，Tice等人曾通过测试与电泳方向垂直的彗星细胞直径来寻找慧头，如今的方法则是首先将整个彗星的光密度调合成与电泳垂直并通过其前缘在慧尾的反射实现对“完整”细胞的估算；其次再通过从整个彗星中减去这一“完整” 细胞得出慧尾的DNA（见表2）。 这种对荧光的一维分析较最初Tice等人所提出的方法更能提升信噪比因此被诸多研究者所采用。虽然慧尾部分的DNA比例本身便是有效的试验参数，但大多数研究者综合考虑了慧尾长度从而得出“尾矩“这一参数。通过类比机械工程学中的术语轴距(Random House, 1987)，尾矩已能在彗星试验中得到应用。该应用让人们认识到短尾中可能包含较高比例的DNA而长尾中则可能包含较低比例的DNA碎片。这两种结果已成为备受关注的检测参数。尾矩可以通过将尾部DNA比例与慧尾长度的一半相乘得到。测量慧尾的方法也已得到应用。1988年Singh 等人从细胞彗星的前沿处开始测量慧尾长度（表2中的l"），1990年Olive等人则是从细胞彗星的预计中心处开始测量慧尾（表2中的/'），这些测量说明在没有DNA尾部的情况下应分别取细胞彗星直径或半径作为慧尾，因此可以说这些完整的细胞具有阳性尾矩。考虑到这一结果会使得对弱诱导效应的分辨和数据分析复杂化，所以我们调整了图像分析程序，使其对慧尾的分析从后沿开始（表2中的l）。另外，由于慧尾形状不一，我们决定在计算慧尾矩的过程中忽略分母2。我们提议慧尾矩应是慧尾长度(从细胞彗星的后沿开始测量)与慧尾DNA比例的共同产物。.表3显示的是时长为2小时的控制数据，该数据是通过中心及后沿尾长测量得出的。 翻译好了。。。。汗啊 根本不知道翻译的是神马东西。。。
再问： 啥也不说了，内牛满面呀！！
再答： 我们对细胞彗星的分解是依据所提出的图像分析法进行的，详见表2。现今用到的所有分析方法均需将彗星分解为慧头与慧尾。 1990年，Tice等人曾通过测试与电泳方向垂直的彗星细胞直径来寻找慧头，如今的方法则是首先将整个彗星的光密度调合成与电泳垂直并通过其前缘在慧尾的反射实现对“完整”细胞的估算；其次再通过从整个彗星中减去这一“完整” 细胞得出慧尾的DNA（见表2）。 这种对荧光的一维分析较最初Tice等人所提出的方法更能提升信噪比因此被诸多研究者所采用。虽然慧尾部分的DNA比例本身便是有效的试验参数，但大多数研究者综合考虑了慧尾长度从而得出“尾矩“这一参数。通过类比机械工程学中的术语轴距(Random House, 1987)，尾矩已能在彗星试验中得到应用。该应用让人们认识到短尾中可能包含较高比例的DNA而长尾中则可能包含较低比例的DNA碎片。这两种结果已成为备受关注的检测参数。尾矩可以通过将尾部DNA比例与慧尾长度的一半相乘得到。测量慧尾的方法也已得到应用。1988年Singh 等人从细胞彗星的前沿处开始测量慧尾长度（表2中的l"），1990年Olive等人则是从细胞彗星的预计中心处开始测量慧尾（表2中的/'），这些测量说明在没有DNA尾部的情况下应分别取细胞彗星直径或半径作为慧尾，因此可以说这些完整的细胞具有阳性尾矩。考虑到这一结果会使得对弱诱导效应的分辨和数据分析复杂化，所以我们调整了图像分析程序，使其对慧尾的分析从后沿开始（表2中的l）。另外，由于慧尾形状不一，我们决定在计算慧尾矩的过程中忽略分母2。我们提议慧尾矩应是慧尾长度(从细胞彗星的后沿开始测量)与慧尾DNA比例的共同产物。.表3显示的是时长为2小时的控制数据，该数据是通过中心及后沿尾长测量得出的。 翻译好了。。。。汗啊 根本不知道翻译的是神马东西。。。