英语翻译MG：孔雀石绿 LMG：隐色孔雀绿The use of an LC–MS–MS system was then

英语翻译
MG：孔雀石绿
LMG：隐色孔雀绿
The use of an LC–MS–MS system was then explored for the confirmation of HPLC analysis results.It was found that sensitivity was greatly improved with preservation of accuracy and preci-
sion found for the HPLC–Vis approach (results not shown).Conversion of LMG in the cation MG using a PbO reactor in-line significantly supports ESI-MS.Exploiting the MRM mode by monitoring a number of product-ion tracks (see Section 2.5),the LOD was found at 0.2 mg/kg in tissues.This is much better than the LOD reported for particle beam LC–MS at 20 mg/kg [16,17],for GC–MS at 5 mg/kg [9,17],but comparable for LC–ESP-MS at 0.4 mg/kg (MG) and 0.5 mg/kg (LMG) [8].Isotope dilution LC–APCI-MS was more sensitive for detection of MG,giving LODs at 0.02 mg/kg (MG) and 0.5 mg/kg (LMG) [10].
The possible interference of dyes,of which several are used in animals as well [17] on the determination of MG and LMG,was investigated.Samples containing 1 mg/ml MG and 1 mg/ml LMG were fortified with either crystal violet,Janus green,methylene blue,methylene green,thionine or toluidine blue each at 100 mg/ ml.The response of samples with and without the addition of non-MG chromophores were compared and showed nil or minor interference (Table 4).Thionine gave a considerable interference at the elution position of LMG at this high spiking level.This colorant is a metabolite of methylene blue and may be used as an antimicrobial agent (antiseptic and antidote) in animals [17].It must be noted however that this dye was not taken through the clean-up procedure,which may have eliminated it from the test sample.To determine the inter-day variation,2-g trout samples were spiked with MG and LMG,stored at -20°C until the day of analysis.Samples were analyzed on six different days showing very similar results even when another investigator executed the analysis (Table 5).However,analysis of variance (ANOVA) of these data sets revealed a significant difference at 95% reliability for the recoveries found at the different days.The SD for the determination of MG and LMG within days was 3 and 5%,respecmals tively.At the other hand,the SD for the determination of MG and LMG between days was 7 and 10%,respectively.
软件翻译的就不要复制粘贴了，

然后用一台LC-MS-MS系统来确认HPLC分析的结果.发现敏感性得到极大的改善,而且保留了HPLC-Vis方法原有的精确度和准确度（结果没有显示）.采用嵌入的氧化铅反应器在阳离子MG中LMG的转化明显地支持ESI-MS.通过监测多个产物离子轨迹（件2.5节）拓展了MRM模式,发现组织中的LOD为0.2mg/kg.这比粒子束LC-MS的LOD为20 mg/kg[16,17]和GC–MS的LOD为5 mg/kg [9,17]的报告要好很多,但是与LC-ESP-MS的0.4 mg/kg(MG)和0.5 mg/kg(LMG)[8]相接近.同位素稀释的LC–APCI-MS法对于检测MG更为敏感,它所给出的LOD值为0.02 mg/kg(MG)和0.5 mg/kg (LMG)[10].
染料（有多种染料也会在动物中使用）对MG和LMG检定的干扰的可能性也得到研究.含有1 mg/ml MG和1 mg/ml LMG的样品分别用结晶紫、Janus绿、亚甲蓝、亚甲绿、劳氏紫或甲苯胺蓝强化（每种100 mg/ml）.比较在添加或没有添加额外的非MG发色基团时样品的反应,发现了零或无的干扰作用（图4）.劳氏紫能够对具有高水平尖峰的LMG洗脱位置产生显著干扰.这种着色剂是亚甲蓝的代谢产物,而且在动物中可以作为一种抗微生物药物来使用（防腐剂或解毒剂)[17].然而,必须要注意这种染料不能通过清洁程序来给予,这可能会已经从检测样本中将其消除了.为了确定日间变异,刺有MG和LMG的2克鲑鱼样本被放置在零下20度储存,直到进行分析的那一天.在不同的六天中分别分析这些样品,发现结果非常相似,甚至包括另一名检测人员完成的分析工作（见表5）.然而,这些数据的变异性分析（ANOVA）揭示出在不同的日子里回收率出现明显的变化（95%的可信度）.确定MG和LMG的标准差在三天内分别为3%和5%.此外,确定MG和LMG的日间标准差分别为7%和10%.