英语翻译求大神翻译为英文:植物叶片经液氮研磨,可使细胞壁破裂,加入去污剂(如CTAB),可使核蛋白体解析,然后使蛋白和多
来源:学生作业帮 编辑:作业帮 分类:英语作业 时间:2024/07/16 14:06:58
英语翻译
求大神翻译为英文:植物叶片经液氮研磨,可使细胞壁破裂,加入去污剂(如CTAB),可使核蛋白体解析,然后使蛋白和多糖杂质沉淀,DNA进入水相,再用酚、氯仿抽提纯化.本实验采用CTAB法,其主要作用是破膜.CTAB是一种非离子去污剂,能溶解膜蛋白与脂肪,也可解聚核蛋白.植物材料在CTAB的处理下,结合 65℃ 水浴使细胞裂解、蛋白质变性、DNA 被释放出来.CTAB与核酸形成复合物,此复合物在高盐(>0.7mM)浓度下可溶,并稳定存在,但在低盐浓度(0.1-0.5mM NaCl)下CTAB-核酸复合物就因溶解度降低而沉淀,而大部分的蛋白质及多糖等仍溶解于溶液中.经过氯仿 / 异戊醇 (24:1) 抽提去除蛋白质、多糖、色素等来纯化DNA,最后经异丙醇或乙醇等沉淀剂将DNA沉淀分离出来.
由于核酸、蛋白质、多糖在特定的紫外波长都有特征吸收.核酸及其衍生物的紫外吸收高峰在260nm.纯的DNA样品A260/280≈1.8,纯的RNA样品A260/280≈2.0,并且1μg/ml DNA溶液A260=0.020.
能有没语病的么,不要直接丢XX翻译,复制黏贴。
求大神翻译为英文:植物叶片经液氮研磨,可使细胞壁破裂,加入去污剂(如CTAB),可使核蛋白体解析,然后使蛋白和多糖杂质沉淀,DNA进入水相,再用酚、氯仿抽提纯化.本实验采用CTAB法,其主要作用是破膜.CTAB是一种非离子去污剂,能溶解膜蛋白与脂肪,也可解聚核蛋白.植物材料在CTAB的处理下,结合 65℃ 水浴使细胞裂解、蛋白质变性、DNA 被释放出来.CTAB与核酸形成复合物,此复合物在高盐(>0.7mM)浓度下可溶,并稳定存在,但在低盐浓度(0.1-0.5mM NaCl)下CTAB-核酸复合物就因溶解度降低而沉淀,而大部分的蛋白质及多糖等仍溶解于溶液中.经过氯仿 / 异戊醇 (24:1) 抽提去除蛋白质、多糖、色素等来纯化DNA,最后经异丙醇或乙醇等沉淀剂将DNA沉淀分离出来.
由于核酸、蛋白质、多糖在特定的紫外波长都有特征吸收.核酸及其衍生物的紫外吸收高峰在260nm.纯的DNA样品A260/280≈1.8,纯的RNA样品A260/280≈2.0,并且1μg/ml DNA溶液A260=0.020.
能有没语病的么,不要直接丢XX翻译,复制黏贴。
植物叶片经液氮研磨,可使细胞壁破裂,加入去污剂(如CTAB),可使核蛋白体解析,然后使蛋白和多糖杂质沉淀,DNA进入水相,再用酚、氯仿抽提纯化.本实验采用CTAB法,其主要作用是破膜.CTAB是一种非离子去污剂,能溶解膜蛋白与脂肪,也可解聚核蛋白.植物材料在CTAB的处理下, 结合 65℃ 水浴使细胞裂解、蛋白质变性、DNA 被释放出来.CTAB与核酸形成复合物,此复合物在高盐(>0.7mM)浓度下可溶,并稳定存在,但在低盐浓度(0.1-0.5mM NaCl)下CTAB-核酸复合物就因溶解度降低而沉淀,而大部分的蛋白质及多糖等仍溶解于溶液中.经过氯仿 / 异戊醇 (24:1) 抽提去除蛋白质、多糖、色素等来纯化DNA,最后经异丙醇或乙醇等沉淀剂将DNA沉淀分离出来.
Plant leaves by grinding in liquid nitrogen, can make the cell wall broken, adding detergent (such as CTAB), can make nuclear protein analysis, and then theprotein and polysaccharide impurity precipitation, DNA into the aqueous phase,and phenol chloroform extraction, purification. This experiment adopts the CTABmethod, its main role is to rupture of membranes. CTAB is a nonionic detergent,can dissolve the membrane protein and fat, can also be depolymerized nuclearprotein. Plant materials in CTAB treatment, combined with 65 ℃ water bath to cell lysis, protein denaturation, DNA is released. CTAB and nucleic acid complexes, thecomplexes in high salt (>0.7mM) concentration and soluble, stable, but at low salt concentration (0.1-0.5mM NaCl) CTAB- nucleic acid complexes because ofreduced solubility and precipitation, and most of the protein and polysaccharide isdissolved in solution. By chloroform / isoamyl alcohol (24:1) extraction to remove proteins, polysaccharides, pigments for purification of DNA, and finally by isopropyl alcohol or ethanol as precipitant DNA precipitation separated.
由于核酸、蛋白质、多糖在特定的紫外波长都有特征吸收.核酸及其衍生物的紫外吸收高峰在260nm.纯的DNA样品A260/280≈1.8,纯的RNA样品A260/280≈2.0,并且1μg/ml DNA溶液A260=0.020.
Because the nucleic acid, protein, polysaccharide has the characteristic absorptionin the UV wavelength specific. Nucleic acid and its derivative UV absorption peak at 260nm. Pure DNA samples with A260/280 ≈ 1.8, pure RNA sample A260/280 ≈ 2,and 1 μ g/ml DNA solution A260=0.020
Plant leaves by grinding in liquid nitrogen, can make the cell wall broken, adding detergent (such as CTAB), can make nuclear protein analysis, and then theprotein and polysaccharide impurity precipitation, DNA into the aqueous phase,and phenol chloroform extraction, purification. This experiment adopts the CTABmethod, its main role is to rupture of membranes. CTAB is a nonionic detergent,can dissolve the membrane protein and fat, can also be depolymerized nuclearprotein. Plant materials in CTAB treatment, combined with 65 ℃ water bath to cell lysis, protein denaturation, DNA is released. CTAB and nucleic acid complexes, thecomplexes in high salt (>0.7mM) concentration and soluble, stable, but at low salt concentration (0.1-0.5mM NaCl) CTAB- nucleic acid complexes because ofreduced solubility and precipitation, and most of the protein and polysaccharide isdissolved in solution. By chloroform / isoamyl alcohol (24:1) extraction to remove proteins, polysaccharides, pigments for purification of DNA, and finally by isopropyl alcohol or ethanol as precipitant DNA precipitation separated.
由于核酸、蛋白质、多糖在特定的紫外波长都有特征吸收.核酸及其衍生物的紫外吸收高峰在260nm.纯的DNA样品A260/280≈1.8,纯的RNA样品A260/280≈2.0,并且1μg/ml DNA溶液A260=0.020.
Because the nucleic acid, protein, polysaccharide has the characteristic absorptionin the UV wavelength specific. Nucleic acid and its derivative UV absorption peak at 260nm. Pure DNA samples with A260/280 ≈ 1.8, pure RNA sample A260/280 ≈ 2,and 1 μ g/ml DNA solution A260=0.020
英语翻译求大神翻译为英文:植物叶片经液氮研磨,可使细胞壁破裂,加入去污剂(如CTAB),可使核蛋白体解析,然后使蛋白和多
CTAB法提取植物DNA,液氮研磨后,添加CTAB提取液这步具体怎样进行啊,
液氮研磨植生物材料液氮研磨植物材料,如叶片,放进研钵倒入液氮,研磨得到干渣,粘在研钵内壁,而一般文献都说得到干粉,为什么
请问用CTAB法提取植物DNA时,用液氮研磨到什么时候差不多.
多少分贝的声音可使玻璃杯破裂
植物细胞分泌蛋白吗?如果植物细胞分泌蛋白的话,应该也是和动物细胞一样通过胞吐出细胞的吧?那细胞膜就会增大,可细胞壁又会束
trizol提取烟草叶片总RNA,在液氮研磨后加入1ml的trizol变成糊状而不是溶液状
液氮研磨植物材料的问题
提取植物组织DNA要液氮研磨
CTAB法提取真菌DNA时,研磨菌丝不用液氮可以吗
英语翻译请为我翻译一下这个短语”可与而不可求“成英文,
植物叶片的结构植物叶片有哪些结构?最好有图(没有也可).