英语翻译Fig.1.The carotenoid and visual pigment content of ninaD

英语翻译
Fig.1.The carotenoid and visual pigment content of ninaDP245 flies is
drastically reduced.(A)HPLC analyses of the lipohilic compounds of the fly’s
head are shown.The two major carotenoids were lutein and zeaxanthin.The
upper lane gives the HPLC profile at 450 nm of an extract from heterozygous
ninaDP245,the lower lane of an extract of homozygous ninaDP245;each extract
was yielded from the heads of 3,000 flies.(B)Total carotenoid contents of
heads and trunks of homozygous ninaDP245 flies and heterozygous controls.
The values give the average of 3,000 flies.(C)Western blot analyses showing
the content and maturation state of the major rhodopsin Rh1 of the flies.
Immature Rh1 migrates as a dimer at 80 kDa,mature Rh1 at 32 kDa.Lane 1,
homozygous ninaDP245 raised on standard corn medium;lane 2,homozygous
ninaDP245 raised on standard corn medium supplemented with-carotene;
lane 3,homozygous ninaDP245 raised on standard corn medium supplemented
with zeaxanthin;lane 4,homozygous ninaDP245 raised on standard corn
medium supplemented with retinal;lane 5,heterozygous ninaDP245 controls.
Fig.3.Expression of a ninaD(wt)transgene restores the vitamin A-deficient
phenotype of ninaDP245 flies.Adult homozygous ninaDP245 flies carrying a
UAS-ninaD(wt)transgene expressed under the control of a hs-GAL4 driver were
compared with heterozygous and non-heat-shocked homozygous control
flies.The flies used had the following genotypes:control,w;ninaDP245 CyO;
;ninaD,w;ninaDP245 ninaDP245;;hs rescue and non-hs control,w;
ninaDP245 ninaDP245;hs-GAL4 UAS-ninaD(wt).hs rescue flies were heat-
shocked for 1 h at 37°C and analyzed 72 h later.For the dietary rescue of ninaD
flies,the corn medium was supplemented with retinal for 3 days.(A)Protein
extracts were subjected to Western blot analysis using an anti-Rh1 antibody.
Mature Rh1 migrated at 32 kDa,immature Rh1 as a dimer at 80 kDa.(B)
Carotenoid contents of the whole flies were determined by HPLC analyses,
and for quantification,known amounts of authentic carotenoid standards
were used.The values give the average of 63 flies per experiment.n.d.,not
detectable.

Fig.1.The类胡萝卜素和视觉ninaDP245苍蝇色素含量急剧减少.（一）的头苍蝇高效液相色谱法分析了lipohilic化合物所示.两个主要的类胡萝卜素叶黄素和玉米黄质.上层行车线给出了一个在450 nm的高效液相色谱专页杂ninaDP245提取,低线的提取纯ninaDP245,每提取,取得了3000头从苍蝇.（三）Western blot分析显示Rh1蝇内容和成熟状态视紫红质主要作为一个在80 kDa的二聚体幼Rh1迁移,成熟Rh1 32 kDa.Lane 1纯ninaDP245中提出的标准玉米;巷2,纯ninaDP245提出有关标准玉米胡萝卜素培养基与-;泳道3,玉米纯合ninaDP245标准中supplementedwith玉米黄质提高;泳道4,纯ninaDP245与视网膜补充标准cornmedium提高;5,杂ninaDP245控制Fig.3.Expression一个ninaD（wt）的转基因恢复的维生素A缺乏ninaDP245表型的苍蝇.
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