英语翻译Caged molecules are substances that include a key functi
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英语翻译
Caged molecules are substances that include a key functionality masked by a protecting group that can be deprotected with the application of an external signal,usually UV light.Upon uncaging,active species that produce specific biological responses can be generated with spatial and temporal control.Fluorescent probes,on the other hand,have found widespread applications because of the potential for imaging biological processes with high selectivity and sensitivity.Of particular interest are the environment-sensitive fluorophores that change spectral properties upon changes in environment.6-Propionyl-2-(dimethylamino)naphthalene (PRODAN),
first introduced by Weber and Farris,is an example of an environmentally sensitive fluorophore.Upon transfer to more hydrophobic environments,there is a marked blue shift in the emission maximum wavelength,and the quantum yield of the PRODAN fluorophore increases.In this report,the PRODANbased amino acid,6-(2-dimethylaminonaphthoyl)alanine (DANA),is used to probe the phosphorylation-dependent binding of an octapeptide to the 14-3-3 ú isoform.5 On the basis of previously reported peptide library screening that identified the optimal sequence for 14-3-3 ú binding and the X-ray structure of 14-3-3 bound with octapeptide 1,we synthesized peptides 2,3,and 4,incorporating DANA and the key serine residue in three forms:unmodified serine,phosphoserine,and caged phosphoserine (Chart 1).Phosphoserine was caged as the 1-(2-nitrophenyl)ethyl derivative.This caging group has been previously used to mask biologically active molecules including divalent calcium,nucleotide derivatives,8 and a variety of peptides and proteins.The photochemical release of these species occurs with reasonable quantum efficiencies at wavelengths above 300 nm.
不要谷歌上的翻译,这我也会……要求通顺,
Caged molecules are substances that include a key functionality masked by a protecting group that can be deprotected with the application of an external signal,usually UV light.Upon uncaging,active species that produce specific biological responses can be generated with spatial and temporal control.Fluorescent probes,on the other hand,have found widespread applications because of the potential for imaging biological processes with high selectivity and sensitivity.Of particular interest are the environment-sensitive fluorophores that change spectral properties upon changes in environment.6-Propionyl-2-(dimethylamino)naphthalene (PRODAN),
first introduced by Weber and Farris,is an example of an environmentally sensitive fluorophore.Upon transfer to more hydrophobic environments,there is a marked blue shift in the emission maximum wavelength,and the quantum yield of the PRODAN fluorophore increases.In this report,the PRODANbased amino acid,6-(2-dimethylaminonaphthoyl)alanine (DANA),is used to probe the phosphorylation-dependent binding of an octapeptide to the 14-3-3 ú isoform.5 On the basis of previously reported peptide library screening that identified the optimal sequence for 14-3-3 ú binding and the X-ray structure of 14-3-3 bound with octapeptide 1,we synthesized peptides 2,3,and 4,incorporating DANA and the key serine residue in three forms:unmodified serine,phosphoserine,and caged phosphoserine (Chart 1).Phosphoserine was caged as the 1-(2-nitrophenyl)ethyl derivative.This caging group has been previously used to mask biologically active molecules including divalent calcium,nucleotide derivatives,8 and a variety of peptides and proteins.The photochemical release of these species occurs with reasonable quantum efficiencies at wavelengths above 300 nm.
不要谷歌上的翻译,这我也会……要求通顺,
Yesterday,those that will be forever
Left phone forgot to delete photos
Pocket was broken as washing tickets
Time to crush all
Like there is any time two of the world
Can not embrace the darkness during the day doomed
We passed the test of how much
Or deadlock
Why does it feel more strongly
It will tear the opposite direction
More injuries were the direct
Why does everything have to go out
Once the heat was just miss the urgent
Why does everything have to incomplete
Just missed a relatively complete
The total time for love not found
Like there is any time two of the world
Can not embrace the darkness during the day doomed
We love the little knowledge
Or burst
Left phone forgot to delete photos
Pocket was broken as washing tickets
Time to crush all
Like there is any time two of the world
Can not embrace the darkness during the day doomed
We passed the test of how much
Or deadlock
Why does it feel more strongly
It will tear the opposite direction
More injuries were the direct
Why does everything have to go out
Once the heat was just miss the urgent
Why does everything have to incomplete
Just missed a relatively complete
The total time for love not found
Like there is any time two of the world
Can not embrace the darkness during the day doomed
We love the little knowledge
Or burst
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are like no other molecules
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