英语翻译Ferric iron is reduced in a reaction with superoxide ani

英语翻译
Ferric iron is reduced in a reaction with superoxide anion and subsequently ferrous
iron is oxidized and thus iron can generate highly reactive
hydroxyl radicals in Fenton reaction.The summary reaction,
referred to as Haber-Weiss reaction,results in
hydroxyl radical production and decrease in superoxide
concentration [15].Low-molecular-mass iron complexes
are also able to produce ROS [16,17].
We have demonstrated previously that iron deprivation
stimulates iron uptake from ferric citrate by human erythroleukemia
K562 cells and that this stimulation depends on
protein synthesis.However,no involvement of any of known
proteins with a suggested role in non-transferrin iron transport
across plasma membrane was detected [18].The existence of
transport system for iron uptake from low-molecular-mass
complexes including ferric citrate was well documented [19–
23].However,specific molecules involved in the transport
system remain obscure in spite of the fact that the identification
of these molecules is highly challenging mainly due to
their potential involvement in some iron disorders.
In order to identify candidate membrane molecules of
the transport system,we employed two-phase partitioning
system which allows the isolation of cell membrane fraction
by a specific manner.Subsequently,we employed
high-resolution 2D electrophoresis and computer analysis
for detection of proteins with increased level in membrane
fraction of K562 cells under iron deprivation.The proteins
with increased level were identified employing liquid
chromatography combined with tandem mass spectrometry.
We have identified two such proteins,i.e.,aldolase A
(ALDA) and voltage-dependent anion channel 2 (VDAC2).
The increased expression of aldolase A and VDAC2 in
K562 cells under iron deprivation was confirmed by
western blot analysis.

铁铁是减少的反应与超氧阴离子自由基,随后有色金属
铁氧化,从而铁可以产生高度反应
羟自由基在Fenton反应.总结反应,
被称为哈伯魏斯反应,结果在
羟自由基的生产和减少超
浓度[ 15 ] .低分子量铁配合
也能够产生活性氧[ 16 ,17 ] .
我们已经证明以前铁剥夺
刺激从铁摄取柠檬酸铁人红
K562细胞,这种刺激取决于
蛋白质的合成.然而,没有参与任何已知的
与蛋白质的作用,建议在非转铁运输
在质膜检测[ 18 ] .存在
交通运输系统的铁摄取低分子量
园区包括柠檬酸铁是有案可稽[ 19 -
23 ] .然而,具体的分子参与运输
系统仍然掩盖,尽管这一事实,即鉴定
这些分子是非常具有挑战性的主要原因
他们的潜力参与一些铁紊乱.
为了确定候选人分子膜
运输系统,我们采用两阶段划分
系统,使孤立细胞膜分数
一个特定的方式进行.随后,我们雇用
高分辨率二维电泳和计算机分析
检测的蛋白质水平增加膜
分数下K562细胞铁剥夺.这些蛋白质
提高水平,确定采用液体
色谱结合串联质谱.
我们已经确定了两个这种蛋白质,即醛甲
（阿尔达）和电压依赖阴离子通道2 （ VDAC2 ） .
增加醛缩酶的表达和在VDAC2
K562细胞铁剥夺下证实了
免疫印迹分析.