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oligo6 中 oligonucleotide stability 与internal stability 含义的区别

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oligo6 中 oligonucleotide stability 与internal stability 含义的区别

Internal stability is calculated with entropy values of neighbor nucleotides.Usually,We draw a graph of ΔG for all nucleotides of the primers.This is known as the stability profile.
Oligonucleotide stability,I guess,may refer to the GC-content of sequence because GC has three hydrogen bond,and is therefore more stable.