英语翻译DLR检测标准流程在进行DLA检测前,LARII ,Stop & Glo® Reagent和样品需要与周

英语翻译
DLR检测标准流程
在进行DLA检测前,LARII ,Stop & Glo® Reagent和样品需要与周围的环境温度平衡.在检测前,请确定LARII 和Stop & Glo® Reagent已经复温到室温.
1.Predispense 100µl of LAR II into the appropriate number of luminometer tubes to complete the desired number of DLR.Assays.
2.Program the luminometer to perform a 2-second premeasurement delay,followed by a 10-second measurement period for each reporter assay.
3.Carefully transfer up to 20µl of cell lysate into the luminometer tube containing LAR II; mix by pipetting 2 or 3 times.Do not vortex.Place the tube in the luminometer and initiate reading.
Note:We do not recommend vortexing the solution at Step 3.Vortexing may coat the sides of the tube with a microfilm of luminescent solution,which can escape mixing with the subsequently added volume of Stop & Glo® Reagent.This is of particular concern if Stop & Glo® Reagent is delivered into the tube by automatic injection.
4.If the luminometer is not connected to a printer or computer,record the
firefly luciferase activity measurement.
5.If available,use a reagent injector to dispense 100µl of Stop & Glo® Reagent.If using a manual luminometer,remove the sample tube from the luminometer,add 100µl of Stop & Glo® Reagent and vortex briefly to mix.Replace the sample in the luminometer,and initiate reading.
6.Discard the reaction tube,and proceed to the next DLR.Assay.

楼上的麻烦不要自动翻译好么,浪费网络资源.
1.分装LARII 100μL到光度计管,你要检验多少管就分装多少管.
2.光度计程序设定成先两秒的延迟,然后10秒的测量.
3.把20μL细胞裂解液转移到有LARII的光度计管里,用枪吹打两三次混匀,不要漩涡振荡.放到光度计开始检测.
Note讲了为什么不要漩涡振荡,不翻译了.
4.如果光度计没有连接打印机或者电脑,自己记下荧光强度的数据.
5.如果可以,用反应注射器添加100μL的Stop & Glo® Reagent.如果没有,是手动的光度计,就把样品拿出来,加100μL的Stop & Glo® Reagent,稍微漩涡振荡一下,然后再放回去读取数据.
6.丢了管子,进行下一个测量.