英语翻译请不要复制!感激不尽~请人工翻译 定重谢！siRNA特征 在目前的RNAi模型中，第一步是Rnase-Ⅲ类的核酸

英语翻译
请不要复制!感激不尽~
请人工翻译 定重谢！
siRNA特征
在目前的RNAi模型中，第一步是Rnase-Ⅲ类的核酸酶Dicer识别和切割
核糖体的大量小RNA进行测序，鉴定出许多来源于两种非LTR类型的反转
录转座子元件——INGI和SLACS中的小RNA序列，它们具有所有siRNA
的特征：具有反转录转座子转录物的正义和负义链;有与肌动蛋白siRNA相
同的大小；也有可溶和多聚体两种组分。尤其重要的是，这些反转录转座子特
异的siRNA在锥虫中是组成型存在的，它与是否通过转基因表达高水平的
dsRNA来激活RNAi无关。这种现象说明，在锥虫中RNAi是一种组成型的
机制，其功能是作为一个质量控制系统来降低有害基因产物表达。与此一致的
在RNAi缺陷的锥虫或TbAGO-/-细胞（见下）中，反转录转座子转录
物的数量与野生型细胞相比增加了3~5倍（H.Shi等，待发表），这为锥虫
RNAi的一个生物学功能是沉默反转录转座子录物，从而抑制或者降低反转
录和它对基因组完整性的有害作用提供了遗传学证据。因为siRNA来源于
dsRNA的剪切，对产生dsRNA的反转录转座子转录物的精确结构还不清楚。

siRNA characteristic in the present RNAi model,the first step is Rnase-ⅢKind of nuclease Dicer distinguishes and cuts ribosome massive small RNA to carry on the sequence,appraises many originates in two kind of non-LTR type reverse records in swivel sub-element - - INGI and the SLACS small RNA sequence,they have all siRNA characteristic:Has reverses transcription the transposable elements duplication justice and is ungrateful the chain; Has with actin siRNA with size; Also has may fuse gathers the body two kind of components.Especially more importantly,these reverse transcription transposable elements different siRNA in the awl insect are especially the composition existence,it with whether to activate RNAi through transgene expression high level's dsRNA to have nothing to do with.This kind of phenomenon showed that in the awl insect RNAi is one kind of composition the mechanism,its function is takes a quality control system to reduce the harmful gene product expression.With this consistent is,in the RNAi flaw's awl insect or the TbAGO-/- cell (sees),reversed transcription the transposable elements to copy the thing quantity and the wild cell compares increased 5 times (H.Shi and so on,treated publication),this was the silence reverses transcription the transposable elements for an awl insect RNAi biology function to record the thing,thus suppressed or reduces reverses to record with it has provided the genetics evidence to the genome team complete adverse effect.Because siRNA originates from the dsRNA cutting,pair process dsRNA reverses transcription the transposable elements duplication precise structure not to be clear.
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