英语翻译Background Few studies have examined the properties of h

英语翻译
Background Few studies have examined the properties of human immunodeficiency virus type 1 (HIV-1) epitope-specific cytotoxic T lymphocyte (CTL) responses in children.To address this issue,we characterized epitope-specific CTL responses and analyzed the determinants that may affect CTL responses before and after highly active antiretroviral therapy (HAART) in children with HIV-1 infection.Methods A total of 22 HIV-1-infected children and 23 uninfected healthy children as control were enrolled in the study.Circulating CD4 T cells and HIV-1 RNA load in plasma were routinely measured.Peripheral HIV-1-specific CTL frequency and HIV-1 epitope-specific,interferon-γ (IFN-γ)-producing T lymphocytes were measured using tetramer staining and enzyme-linked immunospot (ELISPOT) assay,respectively.Circulating dendritic cell (DC) subsets were monitored with FACS analysis.Results More than 80％ of the children with HIV-1 infection exhibited a positive HIV-1-epitope-specific CTL response at baseline,but HIV-specific CTLs and IFN-γ-producing lymphocytes decreased in patients who responded to HAART in comparison with non-responders and HAART-naive children.The duration of virus suppression resulted from HAART was inversely correlated with CTL frequency.While in HAART-naive children,HIV-1-specific CTL frequency was positively correlated with myeloid DC (mDC) frequency,although the cause and effect relationship between the DCs and CTLs remains unknown.Conclusions HIV-1-epitope-specific CTL responses are dependent on antigenic stimulation.The impaired DC subsets in blood might result in a defect in DC-mediated T cell responses.These findings may provide insight into understanding the factors and related mechanisms that influence the outcome of HIV-1 carriers to HAART or future antiviral therapies.

背景少量研究审查了HIV 第一类型(HIV-1) epitope 具体细胞毒素的T 淋巴细胞(CTL) 反应物产对于儿童.论及这个问题, 我们描绘epitope 具体CTL 反应和分析了也许影响CTL 反应的定列式在高度活跃antiretroviral 疗法(前后HAART) 对于儿童以HIV-1 infection.Methods A 共计22 个HIV 1 被传染的孩子和23 个未感染的健康孩子作为控制注册了在研究.流通的CD4 T 细胞和HIV-1 核糖核酸装载在血浆定期地被测量了.周边HIV 1 具体CTL 频率和HIV-1 epitope 具体, 干扰素-? (IFN?) 生产T 淋巴细胞被测量了使用四聚物弄脏和酵素连接了immunospot (ELISPOT) 分析用试样, 树状细胞的respectively.Circulating
(DC) 子集被监测与FACS analysis.Results 超过80? 孩子以HIV-1 传染陈列了一个正面HIV 1 epitope 具体CTL 反应在基础线, 但HIV 具体CTLs 和IFN? 生产淋巴细胞被减少在反应HAART 与非回信者和HAART 天真孩子比较的患者.镇压起因于HAART 病毒的期间相反地被关联了以CTL 频率.当在HAART 天真孩子,
HIV 1 具体CTL 频率正面地被关联了以myeloid DC (mDC) 频率, 虽然起因和作用关系在DCs 和CTLs 遗骸之间的unknown.Conclusions HIV 1 epitope 具体CTL 反应依靠抗原刺激.被削弱的DC 子集在血液也许导致一个瑕疵在DC 斡旋的T 细胞反应.这些研究结果也许提供洞察入了解影响HIV-1 载体结果对HAART 或未来抗病毒疗法的因素和相关机制.