有朋友能给我翻译下面一段话成英文的么?谢谢了~越快越好~
来源:学生作业帮 编辑:作业帮 分类:英语作业 时间:2024/07/08 22:08:19
有朋友能给我翻译下面一段话成英文的么?谢谢了~越快越好~
农杆菌Ti质粒转化植物细胞后,在获得的后代分离群体中,有T-DNA插入的纯合突变体,杂合体变体和野生型.在突变体研究中,需要的材料是纯合突变体,所以必须从分离群体中将纯合突变体鉴定出来.本实验通过三组实验来检测突变体:以拟南芥突变体DNA为模板,以LBb1.3和Primer R为引物,用TaKaRa的普通Taq酶PCR扩增含T-DNA插入的序列; 以Primer F和Primer R为引物,用TaKaRa的普通Taq酶PCR扩增无T-DNA插入的基因组上完整的序列.以LBb1.3、Primer F和Primer R为引物,用TaKaRa的普通Taq酶PCR扩增既含有含T-DNA插入的序列,又含有无T-DNA插入的基因组上完整的序列.
要自己手工翻译的哦,论文里面的,比较重要的,谢谢了~
农杆菌Ti质粒转化植物细胞后,在获得的后代分离群体中,有T-DNA插入的纯合突变体,杂合体变体和野生型.在突变体研究中,需要的材料是纯合突变体,所以必须从分离群体中将纯合突变体鉴定出来.本实验通过三组实验来检测突变体:以拟南芥突变体DNA为模板,以LBb1.3和Primer R为引物,用TaKaRa的普通Taq酶PCR扩增含T-DNA插入的序列; 以Primer F和Primer R为引物,用TaKaRa的普通Taq酶PCR扩增无T-DNA插入的基因组上完整的序列.以LBb1.3、Primer F和Primer R为引物,用TaKaRa的普通Taq酶PCR扩增既含有含T-DNA插入的序列,又含有无T-DNA插入的基因组上完整的序列.
要自己手工翻译的哦,论文里面的,比较重要的,谢谢了~
1、Agrobacterium Ti plasmid into plant cells,isolated groups in access to future generations,there are T-DNA insertion homozygous mutants,heterozygotes and wild-type variants.In mutants,the required material is homozygous mutant,so groups will be separated from the homozygous mutant identified.Through this experiment,three groups of experiments to detect mutant:mutant Arabidopsis thaliana DNA as template,and Primer R LBb1.3 primers,Taq enzyme with a TaKaRa PCR amplification of the ordinary with T-DNA insertion sequence; to Primer F and Primer R as primers,with Ta
2、After agricultural bacillus Ti material particle transformation vegetable cell,in obtains the descendant separates in the community,has the T-DNA insertion to gather the mutant purely,heterozygous body anomalous form and wild.In the mutant research,the material which needs gathers the mutant purely,must therefore gather the mutant from the separation community to appraise purely.This experiment examines the mutant through three groups of experiments:South take plans mustard mutant DNA as the template,as directs the thing take LBb1.3 and Primer R,increases with TaKaRa ordinary Taq enzyme PCR including the T-DNA insertion sequence; As directs the thing take Primer F and Primer R,increases the non-T-DNA insertion with TaKaRa ordinary Taq enzyme PCR on the genome team the complete sequence.Take LBb1.3,Primer F and Primer R as directs the thing,increases with TaKaRa ordinary Taq enzyme PCR both includes including the T-DNA insertion sequence,and includes on the non-T-DNA insertion genome team the
你看下,哪个好~还有上面那个回答的你都看下吧.
2、After agricultural bacillus Ti material particle transformation vegetable cell,in obtains the descendant separates in the community,has the T-DNA insertion to gather the mutant purely,heterozygous body anomalous form and wild.In the mutant research,the material which needs gathers the mutant purely,must therefore gather the mutant from the separation community to appraise purely.This experiment examines the mutant through three groups of experiments:South take plans mustard mutant DNA as the template,as directs the thing take LBb1.3 and Primer R,increases with TaKaRa ordinary Taq enzyme PCR including the T-DNA insertion sequence; As directs the thing take Primer F and Primer R,increases the non-T-DNA insertion with TaKaRa ordinary Taq enzyme PCR on the genome team the complete sequence.Take LBb1.3,Primer F and Primer R as directs the thing,increases with TaKaRa ordinary Taq enzyme PCR both includes including the T-DNA insertion sequence,and includes on the non-T-DNA insertion genome team the
你看下,哪个好~还有上面那个回答的你都看下吧.
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